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anti biotin pe  (Miltenyi Biotec)


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    Miltenyi Biotec anti biotin pe
    a , uLIPSTIC system designed to transfer biotin to recipient cells that directly interact with Sox9-CreER expressing hair follicle stem cells residing within discrete niches in the upper hair follicle (uHF), sebaceous gland and bulge. b, Focusing on immune:SC interactions, representative FACS plot of biotinylated versus unlabelled immune cells in Sox9-uLIPSTIC compared to WT skin. These populations were single-cell sequenced and HF SC-interacting cells were distinguished by <t>barcoded</t> <t>anti-biotin</t> antibodies. c, Uniform Manifold Projection (UMAP) plot showing major immune cell types labelled in green by abundance of uLIPSTIC barcode reads. d, Whole mount Vγ5 immunofluorescence images in a max-projection, depicting DETCs prominence in the interfollicular epidermis (IFE) and in the uHF. Scale bar 30μm. * denote autofluorescence. (e-f) UMAP plots color-coded based on unbiased Seurat clustering analysis of DETCs (e) and uLIPSTIC barcode reads (f) . g, barplot of GeneOntology biological processes enriched in uLIPSTIC + (HF-DETC) or uLIPSTIC neg (IFE-DETC). h, volcano plot showing intensity of gene expression (log2 mean gene expression) and correlation score of each gene with uLIPSTIC barcode reads. Red dots denote transcripts whose differential expression is statistically significant (false discovery rate <0.05). i, Representative transcripts enriched in IFE- or HF-DETC were displayed on DETC UMAP plots, color-coded based expression levels. Five Sox9-uLIPSTIC mice in second telogen were pooled into a single sample for sequencing. Further details on statistics and reproducibility in Methods. See for additional supporting experiments.
    Anti Biotin Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti biotin pe/product/Miltenyi Biotec
    Average 99 stars, based on 560 article reviews
    anti biotin pe - by Bioz Stars, 2026-02
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    Images

    1) Product Images from "Immune cells adapt to distinct stem cell niches to govern tissue homeostasis"

    Article Title: Immune cells adapt to distinct stem cell niches to govern tissue homeostasis

    Journal: bioRxiv

    doi: 10.64898/2026.01.28.701831

    a , uLIPSTIC system designed to transfer biotin to recipient cells that directly interact with Sox9-CreER expressing hair follicle stem cells residing within discrete niches in the upper hair follicle (uHF), sebaceous gland and bulge. b, Focusing on immune:SC interactions, representative FACS plot of biotinylated versus unlabelled immune cells in Sox9-uLIPSTIC compared to WT skin. These populations were single-cell sequenced and HF SC-interacting cells were distinguished by barcoded anti-biotin antibodies. c, Uniform Manifold Projection (UMAP) plot showing major immune cell types labelled in green by abundance of uLIPSTIC barcode reads. d, Whole mount Vγ5 immunofluorescence images in a max-projection, depicting DETCs prominence in the interfollicular epidermis (IFE) and in the uHF. Scale bar 30μm. * denote autofluorescence. (e-f) UMAP plots color-coded based on unbiased Seurat clustering analysis of DETCs (e) and uLIPSTIC barcode reads (f) . g, barplot of GeneOntology biological processes enriched in uLIPSTIC + (HF-DETC) or uLIPSTIC neg (IFE-DETC). h, volcano plot showing intensity of gene expression (log2 mean gene expression) and correlation score of each gene with uLIPSTIC barcode reads. Red dots denote transcripts whose differential expression is statistically significant (false discovery rate <0.05). i, Representative transcripts enriched in IFE- or HF-DETC were displayed on DETC UMAP plots, color-coded based expression levels. Five Sox9-uLIPSTIC mice in second telogen were pooled into a single sample for sequencing. Further details on statistics and reproducibility in Methods. See for additional supporting experiments.
    Figure Legend Snippet: a , uLIPSTIC system designed to transfer biotin to recipient cells that directly interact with Sox9-CreER expressing hair follicle stem cells residing within discrete niches in the upper hair follicle (uHF), sebaceous gland and bulge. b, Focusing on immune:SC interactions, representative FACS plot of biotinylated versus unlabelled immune cells in Sox9-uLIPSTIC compared to WT skin. These populations were single-cell sequenced and HF SC-interacting cells were distinguished by barcoded anti-biotin antibodies. c, Uniform Manifold Projection (UMAP) plot showing major immune cell types labelled in green by abundance of uLIPSTIC barcode reads. d, Whole mount Vγ5 immunofluorescence images in a max-projection, depicting DETCs prominence in the interfollicular epidermis (IFE) and in the uHF. Scale bar 30μm. * denote autofluorescence. (e-f) UMAP plots color-coded based on unbiased Seurat clustering analysis of DETCs (e) and uLIPSTIC barcode reads (f) . g, barplot of GeneOntology biological processes enriched in uLIPSTIC + (HF-DETC) or uLIPSTIC neg (IFE-DETC). h, volcano plot showing intensity of gene expression (log2 mean gene expression) and correlation score of each gene with uLIPSTIC barcode reads. Red dots denote transcripts whose differential expression is statistically significant (false discovery rate <0.05). i, Representative transcripts enriched in IFE- or HF-DETC were displayed on DETC UMAP plots, color-coded based expression levels. Five Sox9-uLIPSTIC mice in second telogen were pooled into a single sample for sequencing. Further details on statistics and reproducibility in Methods. See for additional supporting experiments.

    Techniques Used: Expressing, Immunofluorescence, Gene Expression, Quantitative Proteomics, Sequencing



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    a , uLIPSTIC system designed to transfer biotin to recipient cells that directly interact with Sox9-CreER expressing hair follicle stem cells residing within discrete niches in the upper hair follicle (uHF), sebaceous gland and bulge. b, Focusing on immune:SC interactions, representative FACS plot of biotinylated versus unlabelled immune cells in Sox9-uLIPSTIC compared to WT skin. These populations were single-cell sequenced and HF SC-interacting cells were distinguished by <t>barcoded</t> <t>anti-biotin</t> antibodies. c, Uniform Manifold Projection (UMAP) plot showing major immune cell types labelled in green by abundance of uLIPSTIC barcode reads. d, Whole mount Vγ5 immunofluorescence images in a max-projection, depicting DETCs prominence in the interfollicular epidermis (IFE) and in the uHF. Scale bar 30μm. * denote autofluorescence. (e-f) UMAP plots color-coded based on unbiased Seurat clustering analysis of DETCs (e) and uLIPSTIC barcode reads (f) . g, barplot of GeneOntology biological processes enriched in uLIPSTIC + (HF-DETC) or uLIPSTIC neg (IFE-DETC). h, volcano plot showing intensity of gene expression (log2 mean gene expression) and correlation score of each gene with uLIPSTIC barcode reads. Red dots denote transcripts whose differential expression is statistically significant (false discovery rate <0.05). i, Representative transcripts enriched in IFE- or HF-DETC were displayed on DETC UMAP plots, color-coded based expression levels. Five Sox9-uLIPSTIC mice in second telogen were pooled into a single sample for sequencing. Further details on statistics and reproducibility in Methods. See for additional supporting experiments.
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    a , uLIPSTIC system designed to transfer biotin to recipient cells that directly interact with Sox9-CreER expressing hair follicle stem cells residing within discrete niches in the upper hair follicle (uHF), sebaceous gland and bulge. b, Focusing on immune:SC interactions, representative FACS plot of biotinylated versus unlabelled immune cells in Sox9-uLIPSTIC compared to WT skin. These populations were single-cell sequenced and HF SC-interacting cells were distinguished by barcoded anti-biotin antibodies. c, Uniform Manifold Projection (UMAP) plot showing major immune cell types labelled in green by abundance of uLIPSTIC barcode reads. d, Whole mount Vγ5 immunofluorescence images in a max-projection, depicting DETCs prominence in the interfollicular epidermis (IFE) and in the uHF. Scale bar 30μm. * denote autofluorescence. (e-f) UMAP plots color-coded based on unbiased Seurat clustering analysis of DETCs (e) and uLIPSTIC barcode reads (f) . g, barplot of GeneOntology biological processes enriched in uLIPSTIC + (HF-DETC) or uLIPSTIC neg (IFE-DETC). h, volcano plot showing intensity of gene expression (log2 mean gene expression) and correlation score of each gene with uLIPSTIC barcode reads. Red dots denote transcripts whose differential expression is statistically significant (false discovery rate <0.05). i, Representative transcripts enriched in IFE- or HF-DETC were displayed on DETC UMAP plots, color-coded based expression levels. Five Sox9-uLIPSTIC mice in second telogen were pooled into a single sample for sequencing. Further details on statistics and reproducibility in Methods. See for additional supporting experiments.

    Journal: bioRxiv

    Article Title: Immune cells adapt to distinct stem cell niches to govern tissue homeostasis

    doi: 10.64898/2026.01.28.701831

    Figure Lengend Snippet: a , uLIPSTIC system designed to transfer biotin to recipient cells that directly interact with Sox9-CreER expressing hair follicle stem cells residing within discrete niches in the upper hair follicle (uHF), sebaceous gland and bulge. b, Focusing on immune:SC interactions, representative FACS plot of biotinylated versus unlabelled immune cells in Sox9-uLIPSTIC compared to WT skin. These populations were single-cell sequenced and HF SC-interacting cells were distinguished by barcoded anti-biotin antibodies. c, Uniform Manifold Projection (UMAP) plot showing major immune cell types labelled in green by abundance of uLIPSTIC barcode reads. d, Whole mount Vγ5 immunofluorescence images in a max-projection, depicting DETCs prominence in the interfollicular epidermis (IFE) and in the uHF. Scale bar 30μm. * denote autofluorescence. (e-f) UMAP plots color-coded based on unbiased Seurat clustering analysis of DETCs (e) and uLIPSTIC barcode reads (f) . g, barplot of GeneOntology biological processes enriched in uLIPSTIC + (HF-DETC) or uLIPSTIC neg (IFE-DETC). h, volcano plot showing intensity of gene expression (log2 mean gene expression) and correlation score of each gene with uLIPSTIC barcode reads. Red dots denote transcripts whose differential expression is statistically significant (false discovery rate <0.05). i, Representative transcripts enriched in IFE- or HF-DETC were displayed on DETC UMAP plots, color-coded based expression levels. Five Sox9-uLIPSTIC mice in second telogen were pooled into a single sample for sequencing. Further details on statistics and reproducibility in Methods. See for additional supporting experiments.

    Article Snippet: Biotinylated cells were detected using anti-biotin PE (Bio3-18E7, 1:50 Miltenyi Biotec).

    Techniques: Expressing, Immunofluorescence, Gene Expression, Quantitative Proteomics, Sequencing